Difference between revisions of "Part:BBa K4229047:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Was kindly send to us by the Kerfeld group. From this paper https://doi.org/10.1073/pnas.2116871119
+
The plasmid containing this protein was kindly sent to us by the Kerfeld group.
  
 
===References===
 
===References===
 +
H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.
 +
 +
H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.

Latest revision as of 07:38, 12 October 2022


minimal wiffleball with tags under regulation of lambdapLhybrid promoter and LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 444
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 444
    Illegal NotI site found at 1515
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 444
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 444
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 444
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 842
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein was kindly sent to us by the Kerfeld group.

References

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.

H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.