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Revision as of 06:55, 12 October 2022


His - DocS

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 4 (BBa_K4165264), 6x His-tag (BBa_K4165020), DocS (BBa_K3396000) and T7 terminator (BBa_K731721).

Usage and Biology

DOCs protein is a part of the Snitch system, which binds to the His tag for the purification of the Docs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 45

Modeling

Model “peptide (His_DocS) modeled by TRrosetta model1” Ranked the Third model from our 4 ones with a score 5 out of 6 and values of

cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 0 0.77 96 0 -0.29 -0.27 


                             Figure 1. The 3D structure of His Docs model Displayed on Pymol.

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 



                  Figure 2. this figure shows the results from the transcription and translation code showing 
                 the variation of mRNA and protein concentrations with time compared with the wet lab results.

WetLab Results

Comparison between the mathematical model of dry lab and the wet lab results of His DOC using sonicator 

                  Figure 3. This graph shows the correlation between the mathematical model of the dry lab and the wet lab 
                                                results of the His DOC using sonicator

Comparison between the mathematical model of dry lab and the wet lab results of His DOC using chemical lysis 

                 Figure 4. This graph shows the correlation between the mathematical model of the dry lab and the wet lab 
                                                  results of His DOC using chemical lysis