Difference between revisions of "Part:BBa K4156110"
(→hypoxia induced promoter testing) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K4156110 short</partinfo> | <partinfo>BBa_K4156110 short</partinfo> | ||
| − | + | pPepT-mRFP is a biological brick that expresses mRFP controlled by the pPepT promoter (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156078"> BBa_K4156078 </a></html> ). pPepT-mRFP functions to characterize the performance of the pPepT promoter using a red fluorescent signal. | |
| + | |||
| + | |||
| + | <!-- Add more about the biology of this part here --> | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | < | + | In this biobrick, we used the pPepT promoter to regulate the expression of downstream mRFP and eventually introduced the rrnB T1 and T7Te dual terminators. In addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pPepT promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pPepT promoter to changes in oxygen concentration. |
| + | |||
| + | ===Characterization=== | ||
| + | |||
| + | |||
| + | ==hypoxia induced promoter testing== | ||
| + | |||
| + | We constructed a hypoxia reporter consisting of the hypoxia-inducible promoter pPepT+mRFP.Fig 1 indicates that pPepT induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the hypoxia reporter can work properly. | ||
| + | |||
| + | <html> | ||
| + | <figure style="text-align:center;"> | ||
| + | <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/2-2-5-2.png" alt="control"> | ||
| + | <figcaption><b>Figure 1:</b> Induction of downstream gene mRFP expression under hypoxic and normoxic conditions in different chassis organisms over 48h.</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | |||
| + | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4156110 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4156110 SequenceAndFeatures</partinfo> | ||
Latest revision as of 06:23, 12 October 2022
pPepT-mRFP
pPepT-mRFP is a biological brick that expresses mRFP controlled by the pPepT promoter ( BBa_K4156078 ). pPepT-mRFP functions to characterize the performance of the pPepT promoter using a red fluorescent signal.
Usage and Biology
In this biobrick, we used the pPepT promoter to regulate the expression of downstream mRFP and eventually introduced the rrnB T1 and T7Te dual terminators. In addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pPepT promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pPepT promoter to changes in oxygen concentration.
Characterization
hypoxia induced promoter testing
We constructed a hypoxia reporter consisting of the hypoxia-inducible promoter pPepT+mRFP.Fig 1 indicates that pPepT induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the hypoxia reporter can work properly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 723
Illegal AgeI site found at 835 - 1000COMPATIBLE WITH RFC[1000]