Difference between revisions of "Part:BBa K4441003"

 
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<partinfo>BBa_K4441003 short</partinfo>
 
<partinfo>BBa_K4441003 short</partinfo>
  
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40 ATCTCGATGGAGTGGGTCCCGCATTCTGATGACTTCTGGT
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Sequence used in wetlab experiments: AACAGTTGTCTGGATCCATTTTGTGACATCTGACTGAAAGCTGTA is taken from [1]
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This is the sequence for the Backward Inner Primer (BIP). The BIP consists of a B2 region at the 3'end and a B1c region at the 5'end [2]. The B2 region is complementary to the B2c region of the template sequence. The B1c region is identical to the B1c region of the template sequence [w]. This BIP primer is part of the primer set that detects the WT BRAF: TTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGA
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GTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATT
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GTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG
  
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====Dilutions====
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Storage Primer Concentration: 100 μM
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10X Concentration (Stock): 16 μM
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Volume of 10X primer mix: 10 μL
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1X Concentration (Final): 1.6 μM
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Volume of storage primer needed: 1.6 μL
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Mixed with F3, B3, FIP for a total of 3.6 μL -- Final primer mix 1 μL of final primer mix is used in the LAMP reaction mix. For more information, visit https://2022.igem.org/Team:Washington
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==Citations==
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1. Du, Y., Pothukuchy, A., Gollihar, J. D., Nourani, A., Li, B., & Ellington, A. D. (2017). Coupling sensitive nucleic acid amplification with commercial pregnancy test strips. Angewandte Chemie International Edition, 56(4), 992-996.
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2. Loop mediated isothermal amplification - technote. (n.d.). Retrieved October 11, 2022, from http://www.premierbiosoft.com/tech_notes/Loop-Mediated-Isothermal-Amplification.html
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 05:35, 12 October 2022


BRAF WT BIP

Sequence used in wetlab experiments: AACAGTTGTCTGGATCCATTTTGTGACATCTGACTGAAAGCTGTA is taken from [1]

Usage and Biology

This is the sequence for the Backward Inner Primer (BIP). The BIP consists of a B2 region at the 3'end and a B1c region at the 5'end [2]. The B2 region is complementary to the B2c region of the template sequence. The B1c region is identical to the B1c region of the template sequence [w]. This BIP primer is part of the primer set that detects the WT BRAF: TTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGA GTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATT GTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG

Dilutions

Storage Primer Concentration: 100 μM

10X Concentration (Stock): 16 μM

Volume of 10X primer mix: 10 μL

1X Concentration (Final): 1.6 μM

Volume of storage primer needed: 1.6 μL

Mixed with F3, B3, FIP for a total of 3.6 μL -- Final primer mix 1 μL of final primer mix is used in the LAMP reaction mix. For more information, visit https://2022.igem.org/Team:Washington

Citations

1. Du, Y., Pothukuchy, A., Gollihar, J. D., Nourani, A., Li, B., & Ellington, A. D. (2017). Coupling sensitive nucleic acid amplification with commercial pregnancy test strips. Angewandte Chemie International Edition, 56(4), 992-996.

2. Loop mediated isothermal amplification - technote. (n.d.). Retrieved October 11, 2022, from http://www.premierbiosoft.com/tech_notes/Loop-Mediated-Isothermal-Amplification.html Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]