Difference between revisions of "Part:BBa K4274010"

Revision as of 05:26, 12 October 2022

degQ

degQ gene is pleiotropic regulator gene encoding a 46 amino acid polypeptide in Bacillus subtilis 168. It can regulate the expression of a variety of exocrine enzymes and the production of antibacterial substances. Previous studies have shown that the importance of degQ gene in fengycins production. Therefore, degQ gene was knocked-in Bacillus subtilis 168 together with sfp gene from Bacillus amyloliquefaciens FZB42 (Part: BBa_K4274009).

Usage and Biology

degQ gene (Gene ID: 936547) is pleiotropic regulator gene encoding a 46 amino acid polypeptide in Bacillus subtilis 168. It can regulate the expression of a variety of exocrine enzymes and the production of antibacterial substances. It is reported that increased expression of degQ in B. subtilis 168 results in a 7-10 fold increase in antibiotic production. It is because a T base at position -10 in the promoter region of degQ is mutated into a C, resulting in the inability to express degQ.

In this case, DNA sequence of degQ gene under the regulation of p43 promoter was knocked-in the region of the natural sfp gene of B. subtilis. It was used in the composite part PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS (Part: BBa_K4274035) to realize fengycins’ production in B. subtilis. This can be used for other teams working on fengycin production or research on the antibiotic properties of cyclolipopeptides.

Source

Bacillus subtilis 168

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1]Tsuge K., Ano T., Hirai M., et al. The Genes degQ, pps, and Ipa-8(sfp) Are Responsible for Conversion of Bacillus subtilis 168 to Plipastin Production. Antimicrobial Agents and Chemo. 43(9), 2183-2192 (1999). https://doi.org/10.1128/AAC.43.9.2183.

[2]Gao L., Han J., Liu H., et al. Plipastin and surfactin coproduction by Bacillus subtilis pB2-L and their effects on microorganisms. Antonie van Leeuwenhoek. 110 (3), 1007-1018 (2017). https://doi.org/10.1007/s10482-017-0874-y

[3]Yin Y., Tan W., Wen J., et al. Increasing fengycin production by strengthening the fatty acid synthesis pathway and optimizing fermentation conditions. Biochem. Engineering Journal. 177 (21), 1082 (2021). https://doi.org/10.1016/j.bej.2021.108235

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 ==Usage and Biology==
 degQ gene (Gene ID: 936547) is pleiotropic regulator gene encoding a 46 amino acid polypeptide in <i>Bacillus subtilis</i> 168. It can regulate the expression of a variety of exocrine enzymes and the production of antibacterial substances. It is reported that increased expression of degQ in <i>B. subtilis</i> 168 results in a 7-10 fold increase in antibiotic production. It is because a T base at position -10 in the promoter region of degQ is mutated into a C, resulting in the inability to express degQ.
-<br>In this case, DNA sequence of degQ gene under the regulation of p43 promoter was knocked-in the region of the natural sfp gene of <i>B. subtilis</i>. It was used in the composite part PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS (Part: BBa_K4274035) to realize fengycins’ production in <i>B. subtilis</i>. This can be used for other teams working on fengycin production or research on the antibiotic properties of cyclolipopeptides.
+<br>In this case, DNA sequence of degQ gene under the regulation of p43 promoter was knocked-in the region of the natural sfp gene of <i>B. subtilis</i>. It was used in the composite part PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS (Part: BBa_K4274035) to realize fengycins’ production in <i>B. subtilis</i>. This can be used for other teams working on fengycin production or research on the antibiotic properties of cyclolipopeptides.
 ==Source==