Difference between revisions of "Part:BBa K4193019"

 
 
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<partinfo>BBa_K4193019 short</partinfo>
 
<partinfo>BBa_K4193019 short</partinfo>
  
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extra coding
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used to detect if the riboswitch has desired functions
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<html>
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<head>
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<meta charset="utf-8">
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</head>
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<body>
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<div>
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<img src="https://static.igem.wiki/teams/4193/wiki/model/pet28a-gaba1-l1.png" style="width: 80%;">
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</div>
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</body>
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:30, 12 October 2022


pET28a-GABA1-L1

used to detect if the riboswitch has desired functions

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1062
    Illegal XbaI site found at 1205
    Illegal PstI site found at 299
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1062
    Illegal NheI site found at 1101
    Illegal PstI site found at 299
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1062
    Illegal BglII site found at 1271
    Illegal BamHI site found at 192
    Illegal BamHI site found at 336
    Illegal BamHI site found at 1068
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1062
    Illegal XbaI site found at 1205
    Illegal PstI site found at 299
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1062
    Illegal XbaI site found at 1205
    Illegal PstI site found at 299
    Illegal NgoMIV site found at 1303
    Illegal NgoMIV site found at 2891
    Illegal NgoMIV site found at 3051
    Illegal NgoMIV site found at 6098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3971