Difference between revisions of "Part:BBa K4444002"

 
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__NOTOC__
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<partinfo>BBa_K4444010 short</partinfo>
  
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PhoCl Secrete mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, a furin cut site, PhoCl2c, tri-transmembrane domain, and an ER retention motif. PhoCl Secrete will completely release a protein of interest in response to ultra violet light stimulation.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4444010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4444010 parameters</partinfo>
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Revision as of 04:10, 12 October 2022

PhoCl Shed mRuby

PhoCl Secrete mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, a furin cut site, PhoCl2c, tri-transmembrane domain, and an ER retention motif. PhoCl Secrete will completely release a protein of interest in response to ultra violet light stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1288
    Illegal SapI.rc site found at 73