Difference between revisions of "Part:BBa K4180003"
 
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<h4>
  
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SNF1&#916;381-633aa,<!-- C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)-->
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snf1&#916;381-633aa,<!-- C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)-->
  
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C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br>
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C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br>
  
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Citation: <McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200> <br><br>
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Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200> <br><br>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 03:22, 12 October 2022


snf1Δ381-633aa C-truncated

snf1Δ381-633aa, C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200>

Sequence and Features

;      BBa K4180003 snf1Δ381-633aa
     PCR product on lane 3 compared to
      BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa. PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a          comparison



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]