Difference between revisions of "Part:BBa K4180003"
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| − | + | snf1Δ381-633aa,<!-- C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR)--> | |
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| + | C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br> | ||
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| + | Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200> <br><br> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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[[File: ??put picture link in here?? ]] | [[File: ??put picture link in here?? ]] | ||
[[File: ??put picture link in here?? |200px|thumb|left| ??you can write the command in this part?? ]]--> | [[File: ??put picture link in here?? |200px|thumb|left| ??you can write the command in this part?? ]]--> | ||
| − | [[File:BBa K4180003.png|400px|thumb|left|<h3> | + | [[File:BBa K4180003.png|400px|thumb|left|<h3>;      BBa K4180003 snf1Δ381-633aa<br>     PCR product on lane 3 compared to<br>      BBa K4180002 snf1Δ2-306aa |
<br>        PCR product on lanes 1 and 2 </h3>]] | <br>        PCR product on lanes 1 and 2 </h3>]] | ||
| − | [[File:BBa K4180003-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa | + | [[File:BBa K4180003-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa. PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a          comparison </h3>]] |
Latest revision as of 03:22, 12 October 2022
snf1Δ381-633aa C-truncated
snf1Δ381-633aa,
C-terminus truncated from amino acid 381 to 633 deleting the autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein
Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 528
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]