Difference between revisions of "Part:BBa K4166008"

Latest revision as of 03:01, 12 October 2022

Camps -13

Defective in RNAseH-dependent and independent modes; conformational change

Plasmid Copy Number and Expression - Alma 2022

We created a series of mutants of BBa_J04450 in the origin of replication, and wanted to both quantify the copy number and see the effect the copy number has on gene expression.

We estimate that this plasmid has a decreased copy number - only 133 per cell. Interestingly, this shows intense expression!

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 4: / Line 4: @@
 Defective in RNAseH-dependent and independent modes; conformational change
+== Plasmid Copy Number and Expression - Alma 2022 ==
+We created a series of mutants of BBa_J04450 in the origin of replication, and wanted to both quantify the copy number and see the effect the copy number has on gene expression.
+[[File:T--Alma--pcnPlate.png|700px|center]]
+We estimate that this plasmid has a decreased copy number - only 133 per cell. Interestingly, this shows intense expression!
 <!-- Add more about the biology of this part here