Difference between revisions of "Part:BBa K4472991"
(No difference)

Revision as of 02:50, 12 October 2022


Split ribozyme detecting hcat and expressing GFP

This composite part is build out of the split ribozyme halfs, guide RNAs complementary to the stable endogenous E.coli mRNA hcat and a GFP reporter. We assembled this part using golden gate assembly and transformed it into E.coli DH5alpha.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 331
    Illegal NheI site found at 354
    Illegal NheI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
    Illegal XhoI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1495