Difference between revisions of "Part:BBa K4241804"

 
 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4241804 short</partinfo>
 
<partinfo>BBa_K4241804 short</partinfo>
 +
<partinfo>BBa_K4241804 SequenceAndFeatures</partinfo>
  
 +
===Overview===
 
This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This combines the UTR and terminator for a combined protein generator. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9
 
This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This combines the UTR and terminator for a combined protein generator. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This part is used for the inducible expression of Ulp1 in a high-expression manner. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
 +
<br/>
 +
To use this system, include the coding sequence of the protein between the UTR sequences.
  
−
<!-- -->
+
===Parts that comprise this composite component===
−
<span class='h3bb'>Sequence and Features</span>
+
Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790 <br/>
−
<partinfo>BBa_K4241804 SequenceAndFeatures</partinfo>
+
CvNDI_UTR - BBa_K4241799 <br/>
 +
TRAmy3D_UTR - BBa_K4241800 <br/>
 +
TRAmy3D - BBa_K4241794 <br/>
 +
 
 +
===References===
 +
Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., &amp; Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9
  
  

Latest revision as of 02:25, 12 October 2022


CvNDI1_Protein generator (microalgae)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849

Overview

This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This combines the UTR and terminator for a combined protein generator. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Usage and Biology

This part is used for the inducible expression of Ulp1 in a high-expression manner. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.
To use this system, include the coding sequence of the protein between the UTR sequences.

Parts that comprise this composite component

Nitrogen Starvation Inducible promoter 1 (Microalgae) - Part:BBa_K4241790
CvNDI_UTR - BBa_K4241799
TRAmy3D_UTR - BBa_K4241800
TRAmy3D - BBa_K4241794

References

Shin, J.-H., Choi, J., Jeon, J., Kumar, M., Lee, J., Jeong, W.-J., & Kim, S.-R. (2020). The establishment of new protein expression system using n starvation inducible promoters in chlorella. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69620-9