Difference between revisions of "Part:BBa K4472990"
(No difference)

Revision as of 01:55, 12 October 2022


Split ribozyme detecting kanamycin and expressing GFP

This composite part is build out of the split ribozyme halfs, guide RNAs complementary to kanamycin resistance mRNA and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 331
    Illegal NheI site found at 354
    Illegal NheI site found at 768
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal XhoI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1495