Difference between revisions of "Part:BBa K4060021"
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<partinfo>BBa_K4060021 short</partinfo> | <partinfo>BBa_K4060021 short</partinfo> | ||
| − | a | + | aprN (without natural singal peptide) + pelB. |
| + | pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity. | ||
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Latest revision as of 01:34, 12 October 2022
pelB leader sequence with aprN (without native signal peptide sequence)
aprN (without natural singal peptide) + pelB. pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 67
Illegal NotI site found at 211 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 67
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 349