Difference between revisions of "Part:BBa K4169015"

Revision as of 00:57, 12 October 2022

produce TMADH

After expressing, it'll produce trimethylamine dehydrogenase (TMADH (EC 1.5.99.7)). The enzyme TMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of trimethylamine (TMA) to dimethylamine and formaldehyde:
(CH₃)₃N + H₂O → (CH₃)₂NH + CH₂O +2H⁺ + 2e^-.

Metabolic Pathway

Figure 1.Pathways for trimethylamine metabolism in bacteria.

Protein Molecular Structures

Figure 2.Protein molecular structures of trimethylamine dehydrogenase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 388
Illegal XhoI site found at 1717
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
COMPATIBLE WITH RFC[1000]

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+==Protein Molecular Structures==
 <html>
 <div class = "center"><center><img src = "https://static.igem.wiki/teams/4169/wiki/backword/dma/tmd-dmd-structure/tmadh1-1.png" style = "width:75%"></center><br></div>
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+===Sequence and Features===
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4169015 SequenceAndFeatures</partinfo>