Difference between revisions of "Part:BBa K4201011"
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| − | GFP is a commonly used reporter construct throughout the field of biology. When excited by UV or blue light, GFP will fluoresce green, making it an excellent reporter construct<sup>1</sup>. GFP allows for the survival of the genetically modified plant unlike a GUS reporter, which is lethal to the plant<sup>2</sup>. <br> | + | Green Fluorescent protein (GFP) is a commonly used reporter construct throughout the field of biology. When excited by UV or blue light, GFP will fluoresce green, making it an excellent reporter construct<sup>1</sup>. GFP allows for the survival of the genetically modified plant unlike a GUS reporter, which is lethal to the plant<sup>2</sup>. <br> |
When observing the soybeans with the GFP reporter under UV light, green fluorescence was observed in both the transgenic soybeans and the control without transgenes. Because of this, a GFP reporter was not a good reporter to use in our soybean constructs. This is due to autofluorescence from excitement of molecules that are naturally occurring in plants by UV light. The most common of these molecules are chlorophyll and lignin<sup>3</sup>. | When observing the soybeans with the GFP reporter under UV light, green fluorescence was observed in both the transgenic soybeans and the control without transgenes. Because of this, a GFP reporter was not a good reporter to use in our soybean constructs. This is due to autofluorescence from excitement of molecules that are naturally occurring in plants by UV light. The most common of these molecules are chlorophyll and lignin<sup>3</sup>. | ||
Revision as of 00:50, 12 October 2022
Green Fluorescent Protein
Green Fluorescent protein (GFP) is a commonly used reporter construct throughout the field of biology. When excited by UV or blue light, GFP will fluoresce green, making it an excellent reporter construct1. GFP allows for the survival of the genetically modified plant unlike a GUS reporter, which is lethal to the plant2.
When observing the soybeans with the GFP reporter under UV light, green fluorescence was observed in both the transgenic soybeans and the control without transgenes. Because of this, a GFP reporter was not a good reporter to use in our soybean constructs. This is due to autofluorescence from excitement of molecules that are naturally occurring in plants by UV light. The most common of these molecules are chlorophyll and lignin3.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 228
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 228
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 737