Difference between revisions of "Part:BBa K4239008:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Every Igem restriction sites ( | + | Every Igem restriction sites (EcoRI, XbaI, SpeI and PstI) were removed from the part. |
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===Source=== | ===Source=== | ||
− | fiatlux is made up of fiatluxABE, fiatluxCD and the promoter J23117. | + | <i>fiatlux</i> is made up of <i>fiatluxABE</i>, <i>fiatluxCD</i> and the promoter J23117. |
===References=== | ===References=== | ||
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359. | Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359. |
Latest revision as of 00:39, 12 October 2022
fiatlux genes with their promoter to emit luminescence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5342
Illegal BamHI site found at 50 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2993
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1366
Illegal SapI site found at 3512
Design Notes
Every Igem restriction sites (EcoRI, XbaI, SpeI and PstI) were removed from the part.
Source
fiatlux is made up of fiatluxABE, fiatluxCD and the promoter J23117.
References
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.