Difference between revisions of "Part:BBa K4439007"
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Revision as of 00:14, 12 October 2022
mSA-N[AS]4C-CBD-10xHis
Contents
Abstract
To complete
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protein Characterization
Usage and Biology
- Silk proteins demonstrate interesting mechanical properties such as toughness, strength, lightweight, biodegradability and the possibility to produce different morphologies (fibers, foams, capsules, films). In addition to this, silk proteins comprise a high percentage of the amino acids glycine, serine and alanine which have an intermediate hydrophobicity.
- Green lacewing insects produce two types of silk: one produced by the larvae (cocoon) and the other by adult females (egg-stalk). The adult produced silk acts as a protective shelter and structural support for egg stalks, which are two ideal properties for a waterproof coating for our aerogel.
- In green lacewings, two serine- and glycine-rich silk proteins (Ma1XB1 and Ma1XB2) have been identified, both with highly repetitive core domains and small terminal domains. The core domain’s structure is rich in β-sheets with an approximative sheet-length of four amino acids between turns. These form repeating structural units constituting β-helices which have a significant positive correlation with the proteins’ surface hydrophobicity. A consensus motif for the core domain of Ma1XB2 (named [AS])had already been generated. Furthermore, a recombinant protein constituted by 8 repetitions of this [AS] module had also already been expressed in E. coli.
Modeling
Experiments
Figure 1 | Protein Purification Protocol using Ni-NTA Beads
For more information, check out our Protocols page on our wiki.
Figure 2 | Diagram of Procedure for Silk Biofilm Fabrication
- For more information, check out our Protocols page on our wiki.
Lab's Tips and Tricks
- Purification might prove difficult : elution with EDTA works better but might damage the protein more.
- The silk biofilm can be created even if the recombinant protein is in liquid form.
Results
Expression
To complete
Purification
To complete
Biofilm Fabrication
To complete
Hydrophobicity tests
To complete