Difference between revisions of "Part:BBa K4165225"

 
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Clamp [10] composed of Tau Binding Peptide WWW (BBa_K4165007) , linker length of GGSGGG (BBa_K4165224), Tau Binding Peptide TD28rev  (BBa_K4165006). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
 
Clamp [10] composed of Tau Binding Peptide WWW (BBa_K4165007) , linker length of GGSGGG (BBa_K4165224), Tau Binding Peptide TD28rev  (BBa_K4165006). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
  
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===Usage and Biology===
 
===Usage and Biology===
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According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4165225 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165225 SequenceAndFeatures</partinfo>
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===3D Modeling and Ranking===
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TRRosetta models this composite part with a score of 5 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs).
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/10.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                            Figure 1. The 3D structure of Clamp 10 modeled by TRrosetta
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Latest revision as of 00:09, 12 October 2022


Clamp [10]

Clamp [10] composed of Tau Binding Peptide WWW (BBa_K4165007) , linker length of GGSGGG (BBa_K4165224), Tau Binding Peptide TD28rev (BBa_K4165006). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.


Usage and Biology

According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

3D Modeling and Ranking

TRRosetta models this composite part with a score of 5 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs). 

                            Figure 1. The 3D structure of Clamp 10 modeled by TRrosetta