Difference between revisions of "Part:BBa K4201005:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Design considerations
+
Codon optimization for <i>Glycine max</i> (soybean) is a unique design consideration for all of CU Boulder’s parts originating from amino acid sequences, including our cytoTDS2. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.
  
  
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===Source===
 
===Source===
  
−
Source
+
This taxadiene Synthase sequence originates from <i>Taxus chinensis var. mairei</i>. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.
 +
 
  
 
===References===
 
===References===
 +
1. Zou, D. et al. Production of a novel lycopene-rich soybean food by fermentation with Bacillus amyloliquefaciens. LWT 153, 112551 (2022).
 +
<br> 2. De La Peña, R. & Sattely, E. S. Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).
 +
<br> 3. Xiong, X. et al. The Taxus genome provides insights into paclitaxel biosynthesis. Nat. Plants 7, 1026–1036 (2021).

Revision as of 23:16, 11 October 2022


Cyto-TDS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 898
    Illegal BglII site found at 2211
    Illegal XhoI site found at 488
    Illegal XhoI site found at 755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2311


Design Notes

Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s parts originating from amino acid sequences, including our cytoTDS2. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.


Source

This taxadiene Synthase sequence originates from Taxus chinensis var. mairei. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.


References

1. Zou, D. et al. Production of a novel lycopene-rich soybean food by fermentation with Bacillus amyloliquefaciens. LWT 153, 112551 (2022).
2. De La Peña, R. & Sattely, E. S. Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).
3. Xiong, X. et al. The Taxus genome provides insights into paclitaxel biosynthesis. Nat. Plants 7, 1026–1036 (2021).