Difference between revisions of "Part:BBa K4201004"

 
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<partinfo>BBa_K4201004 short</partinfo>
 
<partinfo>BBa_K4201004 short</partinfo>
  
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description
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CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway. Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the Taxus genus10. We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast, which would require us to add export tags and make our engineering goal more difficult to reach.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:05, 11 October 2022


CrtE-cytoTDS-MBP

CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway. Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the Taxus genus10. We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast, which would require us to add export tags and make our engineering goal more difficult to reach.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 55
    Illegal PstI site found at 3853
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 55
    Illegal PstI site found at 3853
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 671
    Illegal BglII site found at 2209
    Illegal BglII site found at 3256
    Illegal BamHI site found at 4307
    Illegal XhoI site found at 1632
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 55
    Illegal PstI site found at 3853
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 55
    Illegal PstI site found at 3853
    Illegal AgeI site found at 1374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 4487
    Illegal SapI.rc site found at 2430
    Illegal SapI.rc site found at 2636