Difference between revisions of "Part:BBa K4201003:Design"
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===Design Notes=== | ===Design Notes=== | ||
Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our CrtE. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans. | Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our CrtE. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans. | ||
| − | <img src=" | + | <a href="https://imgbb.com/"><img src="https://i.ibb.co/jMYJ3by/CRTE.png" alt="CRTE" border="0"></a><br /><a target='_blank' href='https://imgbb.com/'>share images</a><br /> |
Revision as of 22:56, 11 October 2022
CrtE
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 926
Design Notes
Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our CrtE. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.
<a href="https://imgbb.com/"><img src="
" alt="CRTE" border="0"></a>
<a target='_blank' href='https://imgbb.com/'>share images</a>
Source
This Geranylgeranyl-Pyrophosphate Synthase originates from Pantoea ananatis LMG 20103. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.
References
1. Majer, E., Llorente, B., Rodríguez-Concepción, M. & Daròs, J.-A. Rewiring carotenoid biosynthesis in plants using a viral vector. Sci. Rep. 7, 41645 (2017).
2. De La Peña, R. & Sattely, E. S. Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).