Difference between revisions of "Part:BBa K4365019"

 
 
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<partinfo>BBa_K4365019 short</partinfo>
 
<partinfo>BBa_K4365019 short</partinfo>
  
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Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it.  
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Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it. The α-mating factor pre-pro signal peptide has been previously shown to be able to secrete proteins<ref>Peñas M. M. et al. (1998) Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus, Appl Environ Microbiol. 64(10):4028-34. doi: 10.1128/AEM.64.10.4028-4034.1998. PMID: 9758836; PMCID: PMC106595.</ref>.
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{| class="wikitable" style="margin-left: auto; margin-right: auto; border: none; width:600px;"
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|+ Collection of fungal signal peptides
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|-
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!
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! ID
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! Source
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! Species
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! Prediction coefficient <ref name="signalp">José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z </ref>
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! turboRFP secretion
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|- style="text-align:center;"
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|sp1 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365006 BBa_K4365006] || MPGI || <i>Magnaporthe grisea</i> || 0.9562 || '''Yes'''
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|- style="text-align:center;"
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|sp2 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365007 BBa_K4365007] || RodA || <i>Aspergillus nidulans</i> || 0.9553 || '''Yes'''
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|- style="text-align:center;"
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|sp3 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365008 BBa_K4365008] || HYPI  || <i>Aspergillus fumigatus</i> || 0.9703 || No
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|- style="text-align:center;"
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|sp4 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365009 BBa_K4365009] || SsgA || <i>Metarhizium anisopliae</i> || 0.9126 || No
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|- style="text-align:center;"
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|sp5 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365010 BBa_K4365010] || HCF-4 || <i>Cladosporium fulvum</i> || 0.9126 || '''Yes'''
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|- style="text-align:center;"
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|sp6 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365025 BBa_K4365025] || HCF-5 || <i>Cladosporium fulvum</i> || 0.9234 || NA
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|- style="text-align:center;"
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|sp7 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365026 BBa_K4365026] || CCG-2 || <i>Neurospora crassa</i> || 0.9566 || NA
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|- style="text-align:center;"
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|sp8 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365011 BBa_K4365011] || HCF-1 || <i>Cladosporium fulvum</i> || 0.6850 || '''Yes'''
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|- style="text-align:center;"
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|sp9 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365027 BBa_K4365027] || HCF-2 || <i>Cladosporium fulvum</i> || 0.8144 || NA
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|- style="text-align:center;"
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|sp10 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365012 BBa_K4365012] || HCF-3 || <i>Cladosporium fulvum</i> || 0.7934 || No
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|- style="text-align:center;"
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|sp11 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365013 BBa_K4365013] || SC3 || <i>Schizophyllum commune</i> || 0.6327 || No
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|- style="text-align:center;"
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|sp12 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365014 BBa_K4365014] || ABH3 || <i>Agaricus bisporus</i> || 0.9458 || '''Yes'''
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|- style="text-align:center;"
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|sp13 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365028 BBa_K4365028] || COH1 || <i>Coprinus cinereus</i> || 0.8545 || NA
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|- style="text-align:center;"
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|sp14 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365015 BBa_K4365015] || FBH1 || <i>Pleurotus ostreatus</i> || 0.3367 || '''Yes'''
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|- style="text-align:center;"
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|sp15 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365016 BBa_K4365016] || Aa-PRI2 || <i>Agrocybe aegerita</i> || 0.6356 || '''Yes'''
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|- style="text-align:center;"
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|sp16 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365033 BBa_K4365033] || Hyd-Pt1 || <i>Pisolithus tinctorius</i> || 0.8352 || NA
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|- style="text-align:center;"
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|sp17 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365017 BBa_K4365017] || HFBI || <i>Trichoderma reesei</i> || 0.9851 || '''Yes'''
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|- style="text-align:center;"
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|sp18 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365018 BBa_K4365018] || HFBII || <i>Trichoderma reesei</i> || 0.6862 || '''Yes'''
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|- style="text-align:center;"
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|sp19 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365019 BBa_K4365019] || α-mating factor || <i>Saccharomyces cerevisiae</i> || 0.9988 || '''Yes'''
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|}
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<references />
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<br>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4365019 SequenceAndFeatures</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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Latest revision as of 22:53, 11 October 2022


Alpha mating factor signal peptide from Saccharomyces cerevisiae

Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it. The α-mating factor pre-pro signal peptide has been previously shown to be able to secrete proteins[1].

Collection of fungal signal peptides
ID Source Species Prediction coefficient [2] turboRFP secretion
sp1 BBa_K4365006 MPGI Magnaporthe grisea 0.9562 Yes
sp2 BBa_K4365007 RodA Aspergillus nidulans 0.9553 Yes
sp3 BBa_K4365008 HYPI Aspergillus fumigatus 0.9703 No
sp4 BBa_K4365009 SsgA Metarhizium anisopliae 0.9126 No
sp5 BBa_K4365010 HCF-4 Cladosporium fulvum 0.9126 Yes
sp6 BBa_K4365025 HCF-5 Cladosporium fulvum 0.9234 NA
sp7 BBa_K4365026 CCG-2 Neurospora crassa 0.9566 NA
sp8 BBa_K4365011 HCF-1 Cladosporium fulvum 0.6850 Yes
sp9 BBa_K4365027 HCF-2 Cladosporium fulvum 0.8144 NA
sp10 BBa_K4365012 HCF-3 Cladosporium fulvum 0.7934 No
sp11 BBa_K4365013 SC3 Schizophyllum commune 0.6327 No
sp12 BBa_K4365014 ABH3 Agaricus bisporus 0.9458 Yes
sp13 BBa_K4365028 COH1 Coprinus cinereus 0.8545 NA
sp14 BBa_K4365015 FBH1 Pleurotus ostreatus 0.3367 Yes
sp15 BBa_K4365016 Aa-PRI2 Agrocybe aegerita 0.6356 Yes
sp16 BBa_K4365033 Hyd-Pt1 Pisolithus tinctorius 0.8352 NA
sp17 BBa_K4365017 HFBI Trichoderma reesei 0.9851 Yes
sp18 BBa_K4365018 HFBII Trichoderma reesei 0.6862 Yes
sp19 BBa_K4365019 α-mating factor Saccharomyces cerevisiae 0.9988 Yes
  1. ↑ Peñas M. M. et al. (1998) Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus, Appl Environ Microbiol. 64(10):4028-34. doi: 10.1128/AEM.64.10.4028-4034.1998. PMID: 9758836; PMCID: PMC106595.
  2. ↑ José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]