Difference between revisions of "Part:BBa K4488018"

Revision as of 22:46, 11 October 2022

fuBFP

freeuse Blue Fluorescent Protein, a blue fluorescent protein that fluoresces under long-wave UV.

This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021).

Usage and Biology

File:Insert file name.png

Figure 1. Absorption and emission spectrum for fuBFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 151
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 freeuse Blue Fluorescent Protein, a blue fluorescent protein that fluoresces under long-wave UV.
+This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the Y67H mutation in the fluorophore to create fuBFP. The variant has an excitation (380 nm) and emission peak (450 nm) at a shorter wavelength (figure 1). We have also created a fusion protein of fuBFP connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488021).
 ===Usage and Biology===
+[[File:insert file name.png|centre|thumb|700px| Figure 1. Absorption and emission spectrum for fuBFP.]]