Difference between revisions of "Part:BBa K4488016"

 
 
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<partinfo>BBa_K4488016 short</partinfo>
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freeuse Green Fluorescent Protein-Y, a yellow shifted GFP that fluoresces under blue light.
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This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the S66G mutation in the fluorophore to create fuGFPy. The variant has a yellow-shifted excitation peak (figure 1). We have also created a fusion protein of fuGFPy connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488019).
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===Usage and Biology===
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[[File:fuGFP_variants_spectra.png|centre|thumb|700px| Figure 1. Absorption and emission spectrum for fuGFPy and other fuGFP variants (-b,-a)]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4488016 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4488016 parameters</partinfo>
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Latest revision as of 22:38, 11 October 2022


fuGFPy (Yellow Shifted free-use GFP)

freeuse Green Fluorescent Protein-Y, a yellow shifted GFP that fluoresces under blue light.

This part is a variant of fuGFP (Part:BBa K3814004) which was originally designed to create a GFP variant not protected by patents and able to facilitate any kind of research. Using PCR with degenerate primers we introduced the S66G mutation in the fluorophore to create fuGFPy. The variant has a yellow-shifted excitation peak (figure 1). We have also created a fusion protein of fuGFPy connected to a cellulose-binding domain which allows it to be immobilised on cellulose (Part:BBa K4488019).

Usage and Biology

Figure 1. Absorption and emission spectrum for fuGFPy and other fuGFP variants (-b,-a)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]