Difference between revisions of "Part:BBa K4439007"
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=====Procedure=====
 
=====Procedure=====
  
−
*Step 1: <Prepare Buffers>
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======Step 1: Prepare Buffers======
  
−
**Wash Buffer A (1L)
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*Wash Buffer A (1L)
  
−
***300 mM NaCl
+
. 300 mM NaCl
  
−
***20 mM HEPES pH 7.5
+
. 20 mM HEPES pH 7.5
  
−
***20 mM imidazole (for non specific binding)
+
. 20 mM imidazole (for non specific binding)
  
  

Revision as of 22:16, 11 October 2022

mSA-N[AS]4C-CBD-10xHis


Abstract

To complete

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Protein's Characterization

Description

To complete

Usage and Biology

To complete

Modeling

To complete

Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials
  • 2.5M imidazole stock solution
  • 5M NaCl solution
  • Protease inhibitor tablet
  • Turbonuclease
  • Sonicator
  • 1M HEPES
  • HisPur Ni-NTA beads in 20% ethanol
  • Disposable plastic column
  • Centrifuge going to 20 000 xg
  • 0.30 µm filters
  • Tube of bacterial culture induced with IPTG
Procedure
Step 1: Prepare Buffers
  • Wash Buffer A (1L)

. 300 mM NaCl

. 20 mM HEPES pH 7.5

. 20 mM imidazole (for non specific binding)



Lab's Tips and Tricks

To complete

Results

Expression

To complete

Purification

To complete

Biofilm Fabrication

To complete

Hydrophobicity tests

To complete

References