Difference between revisions of "Part:BBa K4439007"

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	*Tube of bacterial culture induced with IPTG		*Tube of bacterial culture induced with IPTG
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		+	=====Procedure=====
		+
		+	*Step 1: Prepare Buffers
		+
		+	**Wash Buffer A (1L)
		+
		+	***300 mM NaCl
		+
		+	***20 mM HEPES pH 7.5
		+
		+	***20 mM imidazole (for non specific binding)
		+

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Revision as of 22:13, 11 October 2022

mSA-N[AS]4C-CBD-10xHis

Abstract

To complete

Sequence and Features

Protein's Characterization

Description

To complete

Usage and Biology

To complete

Modeling

To complete

Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials

• 2.5M imidazole stock solution

• 5M NaCl solution

• Protease inhibitor tablet

• Turbonuclease

• Sonicator

• 1M HEPES

• HisPur Ni-NTA beads in 20% ethanol

• Disposable plastic column

• Centrifuge going to 20 000 xg

• 0.30 µm filters

• Tube of bacterial culture induced with IPTG

Procedure

• Step 1: Prepare Buffers

• • Wash Buffer A (1L)

• • • 300 mM NaCl

• • • 20 mM HEPES pH 7.5

• • • 20 mM imidazole (for non specific binding)

Lab's Tips and Tricks

To complete

Results

Expression

To complete

Purification

To complete

Biofilm Fabrication

To complete

Hydrophobicity tests

To complete

References