Difference between revisions of "Part:BBa K4439007"
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*2.5M imidazole stock solution | *2.5M imidazole stock solution | ||
| − | + | *5M NaCl solution | |
| − | + | *Protease inhibitor tablet | |
| − | + | *Turbonuclease | |
| − | + | *Sonicator | |
| − | + | *1M HEPES | |
| − | + | *HisPur Ni-NTA beads in 20% ethanol | |
| − | + | *Disposable plastic column | |
| − | + | *Centrifuge going to 20 000 xg | |
| − | + | *0.30 µm filters | |
| − | + | *Tube of bacterial culture induced with IPTG | |
Revision as of 22:12, 11 October 2022
mSA-N[AS]4C-CBD-10xHis
Contents
Abstract
To complete
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protein's Characterization
Description
To complete
Usage and Biology
To complete
Modeling
To complete
Experiments
Adapted Protocols
Protein Purification using Ni-NTA Beads
Materials
- 2.5M imidazole stock solution
- 5M NaCl solution
- Protease inhibitor tablet
- Turbonuclease
- Sonicator
- 1M HEPES
- HisPur Ni-NTA beads in 20% ethanol
- Disposable plastic column
- Centrifuge going to 20 000 xg
- 0.30 µm filters
- Tube of bacterial culture induced with IPTG
Lab's Tips and Tricks
To complete
Results
Expression
To complete
Purification
To complete
Biofilm Fabrication
To complete
Hydrophobicity tests
To complete