Difference between revisions of "Part:BBa K4439007"
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===Adapted Protocols===
 
===Adapted Protocols===
−
To complete
+
 
 +
====Protein Purification using Ni-NTA Beads====
 +
 
 +
Materials
 +
. 2.5M imidazole stock solution
 +
. 5M NaCl solution
 +
. Protease inhibitor tablet
 +
. Turbonuclease
 +
. Sonicator
 +
. 1M HEPES
 +
. HisPur Ni-NTA beads in 20% ethanol
 +
. Disposable plastic column
 +
. Centrifuge going to 20 000 xg
 +
. 0.30 µm filters
 +
. Tube of bacterial culture induced with IPTG
 +
 
 +
 
 +
 
  
 
===Lab's Tips and Tricks===
 
===Lab's Tips and Tricks===

Revision as of 22:06, 11 October 2022

mSA-N[AS]4C-CBD-10xHis


Abstract

To complete

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Protein's Characterization

Description

To complete

Usage and Biology

To complete

Modeling

To complete

Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials . 2.5M imidazole stock solution . 5M NaCl solution . Protease inhibitor tablet . Turbonuclease . Sonicator . 1M HEPES . HisPur Ni-NTA beads in 20% ethanol . Disposable plastic column . Centrifuge going to 20 000 xg . 0.30 µm filters . Tube of bacterial culture induced with IPTG



Lab's Tips and Tricks

To complete

Results

Expression

To complete

Purification

To complete

Biofilm Fabrication

To complete

Hydrophobicity tests

To complete

References