Difference between revisions of "Part:BBa K4245006"

Latest revision as of 21:19, 11 October 2022

hsa-miR-1-3p

This part is the sequence for hsa-miR-1-3p, a miRNA isolated from Homo sapiens. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. More specifically, hsa-miR-1-3p, or miRNA-1-3p, is seen to increase in circulation hours and days prior to a heart attack, even before Troponin T is increased in circulation, and it is related to cardiac-specific muscle lineage (Xiao et al., 2019). As such, utilizing miRNA-1-3p has the potential to forewarn heart attacks by days, making it a useful biomarker.

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245100 and BBa_K4245107, the 3' arm for hsa-miR-1-3p and 5' arm for hsa-miR-1-3p, and as the target sequence for BBa_K4245200 and BBa_K4245201, the hsa-miR-1-3p RCA Padlock Probe and the hsa-miR-1-3p RCT Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Xiao, Y., Zhao, J., Tuazon, J. P., Borlongan, C. V., & Yu, G. (2019). MicroRNA-133a and Myocardial Infarction. Cell transplantation, 28(7), 831–838. https://doi.org/10.1177/0963689719843806

Sequence and Features