Difference between revisions of "Part:BBa K180000:Design"
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+ | [1]'' Proc. Natl. Acad. Sci. USA'', Vol. 80, pp. 21-25 |
Revision as of 14:20, 12 October 2009
Hybrid promoter (trp & lac regulated -- tac pR)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
Source
pMAL-pIII (New England Biolabs N8101S)
References
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25