Difference between revisions of "Part:BBa K4232013"

 
 
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<partinfo>BBa_K4232013 short</partinfo>
 
<partinfo>BBa_K4232013 short</partinfo>
  
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This part codes for the promoter nirB, which is derived from the E. coli genome. This part is useful for the detection of intracellular oxygen concentration
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It is a composite part consisting of nirb and RFP capable of emitting red fluorescence under low oxygen conditions.The specific working principles of nirb show in the '''BBa_K4232002 '''.The red fluorescence under hypoxia conditions is shown in Figure 1.
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[[File:BIT-China-parts-red.jpeg|200px|thumb|middle|'''Figure 1.''' Red fluorescence of nirb]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:42, 11 October 2022


Oxygen concentration is characterized by red fluorescence

It is a composite part consisting of nirb and RFP capable of emitting red fluorescence under low oxygen conditions.The specific working principles of nirb show in the BBa_K4232002 .The red fluorescence under hypoxia conditions is shown in Figure 1.

Figure 1. Red fluorescence of nirb








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 409
    Illegal BglII site found at 1278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 3128
    Illegal AgeI site found at 3240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2396
    Illegal SapI.rc site found at 68
    Illegal SapI.rc site found at 2351