Difference between revisions of "Part:BBa K4165192"

Latest revision as of 19:36, 11 October 2022

Amyloid beta peptide 12 (Aβ 29-42)

This part encodes a part of the Amyloid 𝛽 fragment (29-42) which has the ability to bind to A𝛽 plaques inside the brain.

Usage and Biology

Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acid 29 to 42 of the fibril.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

Modeling

The peptide was modeled by several software (Alphafold-Apptest-Pepfold) and the best model from Apptest scored 1 out of 6 according to our Quality assessment code.

                              Figure 1.: Amyloid-beta (29-42) top model visualized by Pymol.

References

Zhao, Y., Cai, J., Liu, Z., Li, Y., Zheng, C., Zheng, Y., ... & Liu, Y. (2018). Nanocomposites inhibit the formation, mitigate the neurotoxicity, and facilitate the removal of β-amyloid aggregates in Alzheimer’s disease mice. Nano letters, 19(2), 674-683.

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acid 32 to 42 of the fibril.
+Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acid 29 to 42 of the fibril.
 ===<span class='h3bb'>Sequence and Features</span>===
 <partinfo>BBa_K4165192 SequenceAndFeatures</partinfo>
 === Dry Lab Characterization ===