Difference between revisions of "Part:BBa K4388005:Experience"
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<p>Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).</p> | <p>Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).</p> | ||
| − | + | [[File:"A E.coli high quality.png]] | |
| − | Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.</p> | + | |
| + | <p>Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:13, 11 October 2022
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Applications of BBa_K4388005
KCL iGEM 2022
Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).
Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.
User Reviews
UNIQ7ed14c720f4d3534-partinfo-00000000-QINU UNIQ7ed14c720f4d3534-partinfo-00000001-QINU