Difference between revisions of "Part:BBa K3876501:Design"
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Revision as of 18:43, 11 October 2022
mimic miRNA-122
Lambert iGEM 2022: hsa-miR-122-5p
Sequence of hsa-miR-122-5p: UGGAGUGUGACAAUGGUGUUUG
The sequence of hsa-miR-122-5p was taken from miRBase, and we chose the mature sequence as it is found in circulation. No changes were made as these were the target sequence.
References:
Mature sequence hsa-miR-122-5p. Mirbase. (n.d.). Retrieved October 10, 2022, from https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MIMAT0000421
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This mimic RNA will be used as a target to detect in a mimic body fluid.
Source
miRBase: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442