Difference between revisions of "Part:BBa K4165202"
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Figure 3. This graph shows a significant difference between chemical lysis and sonication for His Trim21 (L) DOC, after we had the results we optimized our protocol to use sonication for His Trim21 (L) DOC
 
Figure 3. This graph shows a significant difference between chemical lysis and sonication for His Trim21 (L) DOC, after we had the results we optimized our protocol to use sonication for His Trim21 (L) DOC
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<p style=" font-weight: bold; font-size:14px;"> Pull Down Assay results between His Trim21 Doc and GST COH WWW </p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/pull-down/6x-his-trim21-docs-vs-gst-coh2-www.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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Figure 4. This graphs shows the interaction between His Trim21 DOC and GST COH WWW, it shows a significant interaction between them although being tagged to His and GST
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165202 parameters</partinfo>
 
<partinfo>BBa_K4165202 parameters</partinfo>
 
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Revision as of 18:16, 11 October 2022


Trim-(GGGGS)3-Docs

This parts code for the trim21 E3 ligase having his PRYSPRY domain truncated (BBa_K3396007), fused to type 1 Dockerin module derived from Clostridium thermocellum cellulosome scaffoldin using Glycine serine flexible linker repeated three times to maintain part flexibility needed during target ubiquitination.

source

Synthetic Fusion protein

Sequence and Features

sequence was optimized for E.coli expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 535

References

1-Lytle BL, Volkman BF, Westler WM, Heckman MP, Wu JH. Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain. J Mol Biol. 2001 Mar 30;307(3):745-53. doi: 10.1006/jmbi.2001.4522. PMID: 11273698.


WetLab Results

Transformation of His Trim21 (L) Doc in BL-21 using pGS-21a 

                                   Figure 1. Transformed plate of His Trim21 (L) Doc + pGS-21a

Transformation of His Trim21 (L) Doc in DH-5 alpha using pJET vector 

                                   Figure 2. Transformed plate of His Trim21 (L) Doc + pJET

Comparison between chemical lysis and sonication for His Trim21 (L) DOC

Figure 3. This graph shows a significant difference between chemical lysis and sonication for His Trim21 (L) DOC, after we had the results we optimized our protocol to use sonication for His Trim21 (L) DOC

Pull Down Assay results between His Trim21 Doc and GST COH WWW

Figure 4. This graphs shows the interaction between His Trim21 DOC and GST COH WWW, it shows a significant interaction between them although being tagged to His and GST