Difference between revisions of "Part:BBa K4165097"
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===Modeling===
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1c.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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Revision as of 18:02, 11 October 2022


HTRA Binding Peptide 3

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –8.2 kJ.mol-1 and IC50 of 2.5 ± 0.4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 129.77 4.76 25 75 -6.45183 -5.513969


Modeling


                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol.