Difference between revisions of "Part:BBa K4256177"

Latest revision as of 17:37, 11 October 2022

FNR expression system

The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.Under hypoxic conditions, the [4Fe-4S] cluster, which is stably present in the FNR protein, can prompt the FNR protein to form a homodimer, and this dimer can specifically bind to DNA sites and initiate ccdA expression downstream of the target promoter HIP-1; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is repressed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 651
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 651
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 651
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 651
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 651
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4256177 short</partinfo>
-The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.Under hypoxic conditions, the [4Fe-4S] cluster, which is stably present in the FNR protein, can prompt the FNR protein to form a homodimer, and this dimer can specifically bind to DNA sites and initiate ccdA expression downstream of the target promoter HIF-1; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is repressed.
+The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.Under hypoxic conditions, the [4Fe-4S] cluster, which is stably present in the FNR protein, can prompt the FNR protein to form a homodimer, and this dimer can specifically bind to DNA sites and initiate ccdA expression downstream of the target promoter HIP-1; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is repressed.
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