Difference between revisions of "Part:BBa K4117088:Experience"
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===Applications of BBa_K4117088=== | ===Applications of BBa_K4117088=== | ||
| + | “T7 promoter+ PmrA+ RBS+ PmrB+ rnaB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning. | ||
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| + | Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT. | ||
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| + | Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 and CYP2C19, remaining psychoactive. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:37, 11 October 2022
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Applications of BBa_K4117088
“T7 promoter+ PmrA+ RBS+ PmrB+ rnaB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.
Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.
Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 and CYP2C19, remaining psychoactive.
User Reviews
UNIQ53318178e91d36b6-partinfo-00000000-QINU UNIQ53318178e91d36b6-partinfo-00000001-QINU