Difference between revisions of "Part:BBa K174009:Design"
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein wanted to be localised to the spore coat. Gfp can be added to the end of the final sequence.
+
When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein wanted to be localised to the spore coat.  
−
 
+
  
 +
Gfp can be added between CotC and the protein wanted to be localised.
  
 
===Source===
 
===Source===

Latest revision as of 21:32, 11 October 2009

CotC, Bacillus subtilis spore coat protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When fusing proteins with CotC, the final sequence should start with CotC and be followed by the sequence of the protein wanted to be localised to the spore coat.

Gfp can be added between CotC and the protein wanted to be localised.

Source

Bacillus subtilis

References