Difference between revisions of "Part:BBa K4165256"
Line 17: Line 17:
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-www-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-www-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
−
                                     Figure 2. Transformed plate of GST COH (L) WWW + pGS-21a
+
                                     Figure 1. Transformed plate of GST COH (L) WWW + pGS-21a
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH WWW in DH-5 alpha using pJET vector</p>
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH WWW in DH-5 alpha using pJET vector</p>
 
<html>
 
<html>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-www-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-www-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
−
                                     Figure 3. Transformed plate of GST COH (L) WWW + pJET  
+
                                     Figure 2. Transformed plate of GST COH (L) WWW + pJET  
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH WWW </p>
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH WWW </p>
 
<html>
 
<html>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/coh-www.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/coh-www.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
−
Figure 4. this graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW  
+
Figure 3. this graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165256 parameters</partinfo>
 
<partinfo>BBa_K4165256 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 16:12, 11 October 2022


GST-Coh2-G4S-WWW

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH WWW in BL-21 using pGS-21a vector 

                                    Figure 1. Transformed plate of GST COH (L) WWW + pGS-21a

Transformation of GST COH WWW in DH-5 alpha using pJET vector

                                   Figure 2. Transformed plate of GST COH (L) WWW + pJET

Comparison between chemical lysis and sonication for GST COH WWW

Figure 3. this graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW