Difference between revisions of "Part:BBa K4165255"
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-td28-rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-td28-rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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                                 Figure 3. Transformed plate of GST COH (L) TD28 Rev + pGS-21a
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                                 Figure 1. Transformed plate of GST COH (L) TD28 Rev + pGS-21a
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector </p>
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-td-28-rev-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-td-28-rev-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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                                 Figure 4. Transformed plate of GST COH (L) TD28 Rev + pJET  
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                                 Figure 2. Transformed plate of GST COH (L) TD28 Rev + pJET  
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH TD28 Rev </p>
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH TD28 Rev </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/coh-td28rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/coh-td28rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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Figure 5. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev
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Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165255 parameters</partinfo>
 
<partinfo>BBa_K4165255 parameters</partinfo>
 
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Revision as of 16:11, 11 October 2022


GST-Coh2-G4S- TD28REV

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 689
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH (L) TD28 Rev in BL-21 using pGS-21a vector 

                                Figure 1. Transformed plate of GST COH (L) TD28 Rev + pGS-21a

Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector 

                               Figure 2. Transformed plate of GST COH (L) TD28 Rev + pJET

Comparison between chemical lysis and sonication for GST COH TD28 Rev

Figure 3. this graph shows a difference between chemical lysis and sonication for GST COH TD28 Rev, after we had the results we optimized our protocol to use chemical lysis for GST COH (L) TD28 Rev