Difference between revisions of "Part:BBa K4117222:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
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We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503
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UGT1A3 is important
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===References===
 
===References===

Revision as of 15:59, 11 October 2022


UGT1A3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1266
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503

References