Difference between revisions of "Part:BBa K4162118"
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− | This biobrick was created through overlapping PCR of [https://parts.igem.org/Part:BBa_K4162009 BBa_K4162009](ribozyme+B0_RBS+crtE), [https://parts.igem.org/Part:BBa_K4162013 BBa_K4162013](ribozyme+T7_RBS+crtB), [https://parts.igem.org/Part:BBa_K4162016 BBa_K4162016](ribozyme+T7_RBS+crtI) and [https://parts.igem.org/Part:BBa_K4162019 BBa_K4162019](ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, | + | This biobrick was created through overlapping PCR of [https://parts.igem.org/Part:BBa_K4162009 BBa_K4162009](ribozyme+B0_RBS+crtE), [https://parts.igem.org/Part:BBa_K4162013 BBa_K4162013](ribozyme+T7_RBS+crtB), [https://parts.igem.org/Part:BBa_K4162016 BBa_K4162016](ribozyme+T7_RBS+crtI) and [https://parts.igem.org/Part:BBa_K4162019 BBa_K4162019](ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, the RNA sequences of hammerhead ribozyme conduct self-cleaving, and the polycistronic mRNA transcript is thus co-transcriptionally converted into individual mono-cistrons ''in vivo''. Self-interaction of the polycistron can be nullified and each cistron can initiate translation with comparable efficiency. |
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Revision as of 15:57, 11 October 2022
ribozyme+RBS+CDS module: crtEBIY
Introduction
This biobrick was created through overlapping PCR of BBa_K4162009(ribozyme+B0_RBS+crtE), BBa_K4162013(ribozyme+T7_RBS+crtB), BBa_K4162016(ribozyme+T7_RBS+crtI) and BBa_K4162019(ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, the RNA sequences of hammerhead ribozyme conduct self-cleaving, and the polycistronic mRNA transcript is thus co-transcriptionally converted into individual mono-cistrons in vivo. Self-interaction of the polycistron can be nullified and each cistron can initiate translation with comparable efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2202
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 1811
Illegal AgeI site found at 839 - 1000COMPATIBLE WITH RFC[1000]