Difference between revisions of "Part:BBa K4162118"

Revision as of 15:57, 11 October 2022

ribozyme+RBS+CDS module: crtEBIY

Introduction

This biobrick was created through overlapping PCR of BBa_K4162009(ribozyme+B0_RBS+crtE), BBa_K4162013(ribozyme+T7_RBS+crtB), BBa_K4162016(ribozyme+T7_RBS+crtI) and BBa_K4162019(ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, crtEBIY are separated by hammerhead ribozyme, which can perform self-cleaving. Therefore, the long piece of mRNA it transcribes into will be spontaneously separated into four segments, which correspond to crtE, B, I and Y repectively. The translation process of these four enzymes is independent of each other, and the proportion of the expression products of four enzymes can be accurately adjusted by monitoring the intensity of RBS.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2202
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 1811
Illegal AgeI site found at 839
1000
COMPATIBLE WITH RFC[1000]

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 [[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2021 Fudan]]
-This biobrick was created through standard biobrick assembly of K4162009(ribozyme+B0_RBS+crtE), K4162013(ribozyme+T7_RBS+crtB), K4162016(ribozyme+T7_RBS+crtI) and K4162019(ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, crtEBIY are separated by hammerhead ribozyme, which can perform self-cleaving. Therefore, the long piece of mRNA it transcribes into will be spontaneously separated into four segments, which correspond to crtE, B, I and Y repectively. The translation process of these four enzymes is independent of each other, and the proportion of the expression products of four enzymes can be accurately adjusted by monitoring the intensity of RBS.
+This biobrick was created through overlapping PCR of [https://parts.igem.org/Part:BBa_K4162009  BBa_K4162009](ribozyme+B0_RBS+crtE), [https://parts.igem.org/Part:BBa_K4162013  BBa_K4162013](ribozyme+T7_RBS+crtB), [https://parts.igem.org/Part:BBa_K4162016  BBa_K4162016](ribozyme+T7_RBS+crtI) and [https://parts.igem.org/Part:BBa_K4162019  BBa_K4162019](ribozyme+T7_RBS+crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts farnesyl pyrophosphate to beta-carotene. In this part, crtEBIY are separated by hammerhead ribozyme, which can perform self-cleaving. Therefore, the long piece of mRNA it transcribes into will be spontaneously separated into four segments, which correspond to crtE, B, I and Y repectively. The translation process of these four enzymes is independent of each other, and the proportion of the expression products of four enzymes can be accurately adjusted by monitoring the intensity of RBS.
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