Difference between revisions of "Part:BBa K4365020"
 
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<partinfo>BBa_K4365020 short</partinfo>
 
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Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from <i>Entacmaea quadricolor</i> with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or used for protein detection if fused with another protein. This sequence is codon optimized for expression in <i>Saccharomyces cerevisiae</i>.
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Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from <i>Entacmaea quadricolor</i> with excitation and emission maxima 553 and 574 respectively.It possesses prolonged fluorescence lifetime and high pH-stability, and is fast-maturing TurboRFP is more than twice brighter than DsRed2 <ref>Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062</ref>.
  
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It could be used as a selection marker or used for protein detection if fused with another protein. This sequence is codon optimized for expression in <i>Saccharomyces cerevisiae</i>.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4365020 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4365003 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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[[File:BBa K4365001_anemone.jpg|400px|left|thumb|Picture of the sea anemone Entacmaea quadricolor.]]
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[[File:BBa K4365001_spectra.png|400px|left|thumb|Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. FP database. 187 % brighter than of EGFP.]]
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Latest revision as of 15:53, 11 October 2022


turboRFP (yeast)

Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively.It possesses prolonged fluorescence lifetime and high pH-stability, and is fast-maturing TurboRFP is more than twice brighter than DsRed2 [1].

It could be used as a selection marker or used for protein detection if fused with another protein. This sequence is codon optimized for expression in Saccharomyces cerevisiae.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 683


Usage and Biology

Picture of the sea anemone Entacmaea quadricolor.



Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. FP database. 187 % brighter than of EGFP.



  1. ↑ Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062