Difference between revisions of "Part:BBa K4165253"

 
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<partinfo>BBa_K4165253 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165253 SequenceAndFeatures</partinfo>
  
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===WetaLab Results===
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<p style=" font-weight: bold; font-size:14px;"> Transformation of GST DOC in BL-21 using pGS-21a vector </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-doc-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                  Figure 4. Transformed plate of GST Doc + pGS-21a
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<p style=" font-weight: bold; font-size:14px;"> Transformation of GST DOC in DH-5 alpha using pJET vector </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-doc-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                  Figure 5. Transformed plate of GST DOC + pJET
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<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST DOC </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/gst-doc.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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Figure 6. This graph shows a significant difference between chemical lysis and sonication for GST DOC, after we had the results we optimized our protocol to use chemical lysis for GST DOC
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165253 parameters</partinfo>
 
<partinfo>BBa_K4165253 parameters</partinfo>
 
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Revision as of 15:39, 11 October 2022


GST-DocS

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetaLab Results

Transformation of GST DOC in BL-21 using pGS-21a vector 

                                  Figure 4. Transformed plate of GST Doc + pGS-21a

Transformation of GST DOC in DH-5 alpha using pJET vector 

                                  Figure 5. Transformed plate of GST DOC + pJET

Comparison between chemical lysis and sonication for GST DOC

Figure 6. This graph shows a significant difference between chemical lysis and sonication for GST DOC, after we had the results we optimized our protocol to use chemical lysis for GST DOC