Difference between revisions of "Part:BBa K4117022:Design"

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===References===
 
===References===
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[1]Patilea-Vrana GI, Anoshchenko O, Unadkat JD. Hepatic Enzymes Relevant to the Disposition of (-)-∆9-Tetrahydrocannabinol (THC) and Its Psychoactive Metabolite, 11-OH-THC. Drug Metab Dispos. 2019 Mar;47(3):249-256. doi: 10.1124/dmd.118.085548. Epub 2018 Dec 19. PMID: 30567877; PMCID: PMC6374540.
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[2]Gasse A, Vennemann M, Köhler H, Schürenkamp J. Toxicogenetic analysis of Δ9-THC-metabolizing enzymes. Int J Legal Med. 2020 Nov;134(6):2095-2103. doi: 10.1007/s00414-020-02380-3. Epub 2020 Jul 25. PMID: 32712703; PMCID: PMC7578149.
 +
[3]Doohan, P.T., Oldfield, L.D., Arnold, J.C. et al. Cannabinoid Interactions with Cytochrome P450 Drug Metabolism: a Full-Spectrum Characterization. AAPS J 23, 91 (2021). https://doi.org/10.1208/s12248-021-00616-7

Revision as of 15:33, 11 October 2022


CYP2C19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
    Illegal NotI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1395
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P11712

References

[1]Patilea-Vrana GI, Anoshchenko O, Unadkat JD. Hepatic Enzymes Relevant to the Disposition of (-)-∆9-Tetrahydrocannabinol (THC) and Its Psychoactive Metabolite, 11-OH-THC. Drug Metab Dispos. 2019 Mar;47(3):249-256. doi: 10.1124/dmd.118.085548. Epub 2018 Dec 19. PMID: 30567877; PMCID: PMC6374540. [2]Gasse A, Vennemann M, Köhler H, Schürenkamp J. Toxicogenetic analysis of Δ9-THC-metabolizing enzymes. Int J Legal Med. 2020 Nov;134(6):2095-2103. doi: 10.1007/s00414-020-02380-3. Epub 2020 Jul 25. PMID: 32712703; PMCID: PMC7578149. [3]Doohan, P.T., Oldfield, L.D., Arnold, J.C. et al. Cannabinoid Interactions with Cytochrome P450 Drug Metabolism: a Full-Spectrum Characterization. AAPS J 23, 91 (2021). https://doi.org/10.1208/s12248-021-00616-7