Difference between revisions of "Part:BBa K4509420:Design"
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===Characterization=== | ===Characterization=== | ||
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Revision as of 15:27, 11 October 2022
HORSERADISH PEROXIDASE with constitutive promoter J23116
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]
Source and Improvement
The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden in the cell during overexpression of enzymes
