Difference between revisions of "Part:BBa K4195068"
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Ribozyme ENabled Detection of RNA (RENDR) | Ribozyme ENabled Detection of RNA (RENDR) | ||
| − | RENDR is a high-performing, plug-and-play RNA-sensing platform (''1''). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form. | + | RENDR is a high-performing, plug-and-play RNA-sensing platform (''1''). RENDR utilizes a split variant of the ''Tetrahymena thermophila'' ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form. |
[[File:T--XMU-China--RENDR.png|300px]] | [[File:T--XMU-China--RENDR.png|300px]] | ||
'''Fig. 1. Schematic of RENDR that uses an RNA input to template a split ribozyme.''' | '''Fig. 1. Schematic of RENDR that uses an RNA input to template a split ribozyme.''' | ||
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===Usage=== | ===Usage=== | ||
Revision as of 15:01, 11 October 2022
HP14
Stability hairpin of 14nt.
Biology
Ribozyme ENabled Detection of RNA (RENDR) RENDR is a high-performing, plug-and-play RNA-sensing platform (1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
Fig. 1. Schematic of RENDR that uses an RNA input to template a split ribozyme.
Usage
It is added on the upstream of RBS by the standard assembly in RENDR system to improve the efficiency of translation.
Reference
1. L. Gambill et al., https://www.biorxiv.org/content/10.1101/2022.01.12.476080v1 (2022).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]